水木未来（北京）科技有限公司 | SPA项目预研问题清单

SPA项目预研问题清单

Evaluation table of structure determination by single particle method

样品类型（如膜蛋白，可溶蛋白，核酸等）Sample type （Membrane protein, soluble protein, nucleic acid, etc） *

样品毒性（如有传染性活病毒，毒素蛋白等）Gene name (optional)

样品稳定性及组分完整性（可附SEC或胶图）

Buffer constituent

Molecular weight（kD）

Symmetry

Homologous structures

Sample purity (%)

Sample concentration (mg/ml or uM)

sample amout (ug or ul)

Target resolution (Å)

expected time

Expected cost

Others

Note: Minimum sample requirement for negative stain (for homogeneity analysis): 100nM，20uL; Minimum sample requirement for Cryo-EM (for high resolution): >1uM，20uL;

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